Coupling to chiral electrokinetic chromatography: Quenched phosphorescence detection of methotrexate
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منابع مشابه
Quenched phosphorescence as alternative detection mode in the chiral separation of methotrexate by electrokinetic chromatography
Quenched phosphorescence was used, for the first time, as detection mode in the chiral separation of methotrexate (MTX) enantiomers by electrokinetic chromatography. The detection is based on dynamic quenching of the strong emission of the phosphorophore 1-bromo-4-naphthalene sulfonic acid (BrNS) by MTX under deoxygenated conditions. The use of a background electrolyte with 3 mg/mL 2-hydroxypro...
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